FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49203194
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1266960.25749547885041774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT245250.027.3938945
AGACCGC294250.023.0387864
CAGACCG300550.022.5859853
AGAACGA302750.022.2579744
GACCGCG335050.020.2131375
ATACGGA337150.019.98027833
AATACGG339450.019.81838632
TACGGAG338850.019.79371834
AAGAACG341800.019.7084643
ACCGCGT348450.019.467936
CCGCGTT350050.019.3725197
CGCGTTC355450.019.1668288
CAATACG355250.019.08261931
TCAGACC362800.019.0517272
CTCAGAC396400.018.3205641
ACGGAGA368350.018.2451535
CCCAATA374950.018.10447529
GCGTTCT378900.017.9984159
CGGAGAG355450.017.37547736
CCCCAAT404900.016.932128
Produced by FastQC (version 0.11.5)