FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49203194
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG509120.10347295746694818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG178150.014.28802845
GTCACTC250950.014.1424898
GGTCACT253150.013.8414467
ATAGGGG186000.013.71588538
GGCATAG186050.013.61414935
GGGAGGT288400.012.9959393
ACTCTCC274450.012.89034411
GAGCTTA209500.012.26810744
GTTGTCG22650.012.21537526
CATAGGG209750.012.15210937
AGGTCAC302400.012.1469556
TGGCATA210300.012.09776434
GAGGTCA309400.011.9382275
TCACTCT301700.011.9201799
GCATAGG222050.011.48801536
GGAGGTC324700.011.3973134
CACTCTC309500.011.39435510
TCAGACG95850.010.5683972
AGCATAG95800.010.2662445
GCTCTGT357550.010.06645322
Produced by FastQC (version 0.11.5)