FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45640181
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC94550.015.3960515
AGCTTAG139600.014.576406545
GGCATAG147100.013.76760335
TCACCCG112200.013.7351193
ATAGCTC109400.013.00148539
GTTGTCG23300.012.93799526
GAGCTTA162250.012.52767544
ATAGGGG167000.012.39755338
GGTCACT222100.012.2681567
CACCCGG125450.012.1413914
CATAGGG167900.012.08905637
GTCACTC225900.012.0020268
CGGACAG120650.011.97087219
CCGGACA130900.011.87726412
TGGCATA173700.011.7887634
ACACGGA124900.011.59955916
TAGCTCT123600.011.48969240
GCATAGG178700.011.35822436
GGGAGGT278500.011.3255043
CACGGAC129950.011.23534717
Produced by FastQC (version 0.11.5)