FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48116425
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG564350.11728843113344352No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG487580.10133338044129422No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA486000.10100501024338364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC89750.014.5936965
AGCTTAG200400.014.40477945
GGGAGGT333450.014.1467963
GGCATAG203650.014.02748535
ATAGGGG217500.013.35328438
GAGCTTA216200.013.24804344
GGTCACT332900.013.2421067
GGAGGTC344150.012.9869954
TGGCATA222850.012.91996934
TCACCCG103550.012.823293
GTCACTC347450.012.6335248
ATAGCTC97700.012.61883639
ACTCTCC355650.012.47656711
CGGACAC107200.012.179728513
TAGCTCT104850.011.8223540
CCGGACA112450.011.8202612
ACACGGA107450.011.75288616
CGGACAG109850.011.70103119
ACGGACA114550.011.59431318
GACACGG112250.011.53121415
Produced by FastQC (version 0.11.5)