FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46734946
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT986190.2110176825709823No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG599500.128276600554968No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG470000.10056714305393655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC235900.021.629724
CAGACCG248900.020.6631453
GACCGCG245200.020.6624975
ACCGCGT249100.020.3388236
CCGCGTT261400.019.3731887
CGCGTTC266150.019.018478
TTCGACG13000.018.6900986
GAACGAT279050.018.12825645
CGTTACG27650.018.0639321
TCAGACC293500.017.9679432
GCGTTCT296050.017.0825129
CTCAGAC338400.016.9571651
CGAACGA45650.016.31320432
ACGAACG46850.016.18292631
AGAACGA322650.015.73463444
CCGTACG51200.015.5989084
CCGGCTT249000.015.5951762
CGTACGC52750.015.5670135
GTCCGTT28350.015.563841
CTCCGAT39950.015.4400071
Produced by FastQC (version 0.11.5)