FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46734946
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA618080.13225221229527045No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG530450.11350178943183116No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG487920.10440153284867389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT252950.015.2459783
GGAGGTC262000.014.6523274
ATAGGGG204100.013.77847138
GGCATAG204500.013.54258335
AGCTTAG215900.013.12952745
GTTGTCG24200.012.92302226
GAGCTTA224750.012.48250344
TGGCATA225450.012.4741534
ACTCTCC330600.011.58982711
TGGGAGG256700.011.5599172
GGTCACT331500.011.2675687
GTCACTC343500.011.0765228
TCAGACG85550.011.0270462
CATAGGG261900.010.69446337
CTGGGAG325200.010.5025891
GCATAGG270050.010.471736
AGGGATC159000.010.4501952
CGTGGCG126300.010.3103221
GCTCTGT378900.010.0467622
CACCCGG72300.09.9403141
Produced by FastQC (version 0.11.5)