FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44932048
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG469110.10440432183282632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT247800.013.4387273
AGCTTAG176050.012.98434745
ATAGGGG179650.012.86263138
GGCATAG180650.012.6788435
GGTCACT257400.012.4116277
GTCACTC260250.012.3448668
GGAGGTC282300.011.8438024
ACTCTCC286000.011.406219511
GAGCTTA202600.011.32715244
TGGCATA205850.011.2142234
GTTGTCG18750.010.68033926
TCACTCT307550.010.5779739
GAGGTCA320350.010.4716555
CATAGGG221750.010.4206437
CACTCTC308200.010.38751110
TGGGAGG248100.010.0618532
GCATAGG232500.09.97750336
AGGGATC131050.09.3096962
GCTCTGT355850.09.09856122
TAGGGGA355800.08.91016839
Produced by FastQC (version 0.11.5)