FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48532335
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG640560.13198623144754937No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG578480.11919475953506048No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA548590.1130359790024527No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG505450.10414705989316195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG211350.015.70249145
GGCATAG218950.015.11606935
ATAGGGG227700.014.87087638
GGGAGGT320600.014.3756523
TGGCATA239500.013.89420934
GTCACTC321750.013.4681658
GAGCTTA246900.013.45949544
GGTCACT323200.013.4295127
GGAGGTC337600.013.317274
CATAGGG263650.012.7153137
ACTCTCC341400.012.70585511
GTTGTCG27150.012.34764226
GAGGTCA370900.012.1879085
GCATAGG274000.012.18578736
CACTCTC365950.011.85347510
TGGGAGG301950.011.7478862
AGGGATC167050.011.5470992
ACCCGGC71350.011.4792495
TCACTCT384000.011.4310129
CTGGGAG361250.011.0292751
Produced by FastQC (version 0.11.5)