FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53061076
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1236080.23295419037488047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT257900.022.83123245
AGACCGC287650.020.7666324
ACCGCGT293800.020.3242576
CAGACCG297050.020.276193
GACCGCG294300.020.2515015
CCGCGTT299950.019.9147767
CGCGTTC300500.019.8635038
TTCGACG28650.019.4756776
TCAGACC335850.018.0611152
AGAACGA328900.018.02625844
AATACGG340550.017.33691432
ATACGGA345950.017.0791333
GCGTTCT352900.017.0290269
TACGGAG347450.016.92105134
CGGTATA41900.016.64593920
CGTTACG22950.016.56779121
CAATACG361850.016.37888131
AAGAACG369500.016.05174443
CTCAGAC409100.015.9026261
ACGGAGA376750.015.64691435
Produced by FastQC (version 0.11.5)