FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53061076
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG597000.11251185332163259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG30350.014.60655726
GGGAGGT339850.012.62801553
AGCTTAG231800.012.57274945
GGAGGTC352800.011.9797894
GGCATAG247000.011.82579535
ATAGGGG251800.011.7540838
TGGCATA266700.010.98600434
GAGCTTA268350.010.92795544
GGTCACT375950.010.9196097
GTCACTC397050.010.5942748
ACTCTCC398750.010.549643511
ATATAGC54050.010.153436
ACCCGGC75100.09.9734515
TGTCGTT45800.09.87599328
TGGGAGG319200.09.8439672
GAGGTCA446300.09.6764385
CACTCTC437400.09.56586210
CATAGGG312100.09.41775837
GCTAAGT48650.09.38681514
ATAGCTC76200.09.30410539
Produced by FastQC (version 0.11.5)