FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56115849
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1890960.33697431896646524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT329700.028.99458545
GACCGCG387150.024.743755
ACCGCGT391100.024.5111036
AGACCGC392400.024.429924
AGAACGA393850.024.36338844
CAGACCG397450.024.1874393
CCGCGTT402300.023.8007327
CGCGTTC404300.023.688568
TCAGACC449950.021.7202822
AAGAACG449800.021.28296343
AATACGG453200.021.04122232
TACGGAG454250.020.9513934
ATACGGA456400.020.89862333
TTCGGCG72100.020.75571644
GCGTTCT465850.020.7422479
CTCAGAC515500.020.0749631
CAATACG478250.019.9864131
ACGGAGA485000.019.59097335
CGGAGAG492900.019.14003436
CCCAATA506150.019.03634529
Produced by FastQC (version 0.11.5)