FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56115849
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG149550.013.07544938
AGCTTAG150550.013.02783245
GGCATAG154300.012.62790835
GGGAGGT288200.011.1272233
GAGCTTA179650.011.12124944
GTTGTCG25000.010.97889426
GTCACTC283950.010.8811998
TGGCATA182200.010.81499334
CATAGGG182650.010.7424637
GGAGGTC296650.010.5527344
GGTCACT286000.010.5197857
ACTCTCC304450.010.24371111
GCATAGG196150.09.93367236
ATAGCTC75550.09.67994739
ACCCGGC83950.09.65066055
GCGTAGG109500.09.57529236
CGTAGGG110600.09.43995337
GGCGTAG111300.09.42041835
TAGGGGA330700.09.12470139
AGGGATC155000.09.0015832
Produced by FastQC (version 0.11.5)