FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48259739
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1810590.37517608621961257No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG594970.12328496016109827No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA526390.1090743569914458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT317100.026.63919645
AGACCGC378200.022.480614
CAGACCG385850.022.1107223
ACCGCGT389400.021.861696
CCGCGTT390900.021.7899677
GACCGCG389900.021.7709585
CGCGTTC393250.021.7039478
AGAACGA391150.021.68230444
ATACGGA413750.020.47166333
AATACGG413650.020.46568932
TACGGAG416600.020.32070734
TCAGACC423750.020.3190272
AAGAACG428750.019.77579343
CAATACG432150.019.683331
GCGTTCT438750.019.4941849
TTCGACG22850.019.4009656
ACGGAGA442150.019.14645835
AACGCTA166050.019.00668523
CTCAGAC483150.018.8922461
ACGCTAC168850.018.75811624
Produced by FastQC (version 0.11.5)