FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48259739
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG515280.10677223098947966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG184600.013.34623445
ATAGGGG196850.012.52703538
GGGAGGT280250.012.4849483
GGCATAG198000.012.4315535
GTTGTCG22750.012.16481326
GGAGGTC295550.011.8005614
TGGCATA214350.011.5357934
GAGCTTA218150.011.33486944
GGTCACT307650.011.029267
ACTCTCC323250.010.747045511
CATAGGG234000.010.66323237
AGGGATC145150.010.5722952
GTCACTC330050.010.4168868
GCATAGG247550.010.12500936
CACTCTC356100.09.92005110
TCAGACG91650.09.7467542
TGGGAGG282500.09.47832
TAGCGTA6650.09.4734837
CAGGGAT188200.09.4452011
GCGTTCG22000.09.40895715
Produced by FastQC (version 0.11.5)