FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47889659
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG147200.013.81589745
ATAGGGG148950.013.69885738
GGCATAG153900.013.198853535
GTTGTCG22700.012.584022526
GGGAGGT258850.012.485223
GTCACTC254800.012.1978428
ACTCTCC256800.012.05991311
GGTCACT254800.012.0391337
GAGCTTA175300.011.58908944
CATAGGG184800.011.15014737
TGGCATA183150.011.11549434
CACTCTC282400.010.99054410
TCACTCT294250.010.8381129
GGAGGTC302100.010.6006664
AGGTCAC309350.010.4046846
GCATAGG195500.010.40180736
GAGGTCA315450.010.15910155
AGCATAG99550.09.83029345
CGTAACG11950.09.60139642
ATATAGC45400.09.2645676
Produced by FastQC (version 0.11.5)