FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51103421
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG116750.013.28551445
ATAGCTC93850.012.94443439
ACCCGGC100900.012.7104635
GGCATAG123200.012.43728435
TAGCGTA5500.011.86381737
TAGCTCT103400.011.79151440
ATAGGGG135450.011.44432538
CATAGGG134950.011.40439837
TCACCCG125400.010.8869943
TCAGACG87850.010.7747032
TTGATAG116950.010.697041536
ACACGGA118800.010.68064116
TGATAGC118300.010.51791237
GTCACTC225750.010.5078118
GCATAGG147100.010.49302136
GGTCACT227550.010.434137
GAGCTTA150850.010.38744844
ACTCTCC232950.010.3084611
TGGCATA153550.010.15496634
CCGGACA134450.010.11032912
Produced by FastQC (version 0.11.5)