FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45317894
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG675870.14913976364391512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG227550.014.84357945
GGGAGGT343650.014.7462933
GGAGGTC350750.014.2424374
ATAGGGG259450.013.198838
GGCATAG261100.012.97658435
GAGGTCA389800.012.9022255
GTCACTC387150.012.7749938
ACTCTCC391800.012.70358411
GAGCTTA270350.012.64346744
AGGTCAC401250.012.5283216
CACTCTC406450.012.1406210
TCACTCT414400.012.1141269
TGGCATA281400.012.0953234
TGGGAGG301050.012.0247732
GGTCACT427250.011.4282287
GTTGTCG24800.011.33842426
CTGGGAG369400.010.66571
TCTCCCC478200.010.4598613
CATAGGG332200.010.34889137
GCTCTGT485400.010.31613222
Produced by FastQC (version 0.11.5)