FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48319544
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG609250.1260876965229639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT223650.018.1356936
AGACCGC224400.018.0863954
CAGACCG230950.017.6708583
GACCGCG228750.017.6431925
CGCGTTC232950.017.4013968
CCGCGTT236300.017.16477
TCACGCT223700.016.0795758
ACGCGTC25500.016.0554340
GCACTAC94700.015.87639225
TCAGACC274050.015.3924752
CCGGCTT257050.015.1063722
GTTCCTA84200.015.04137215
GCGTTCT272800.014.9171639
GCCGGCT225700.014.8628291
CCCAGAT185700.014.8052141
ACGCTCA254950.014.77036110
TTCACGC248650.014.6112797
CCGTACG52000.014.4937184
CGTTACG10550.014.29280721
CTCAGAC331600.014.2210661
Produced by FastQC (version 0.11.5)