FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54267796
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT555750.1024088024507205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG50450.024.9745756
CGGTATA70250.022.22722220
CGACGAG57700.020.6661198
GTTCGAC58450.020.1754515
AGACCGC129550.019.942494
CAGACCG134750.019.5236513
TCGACGA66550.019.0002577
GACCGCG138700.018.6269595
CCGCGTT139700.018.5374577
ACCGCGT141600.018.2887216
CGCGTTC141700.018.2752918
CGTTACG19400.017.39684121
GAACGAT150550.017.39518545
AGTTCGA74350.016.3451044
ATGACGG100500.015.13353116
TCAGACC179750.015.0240732
ACGAGGA100950.014.88774710
AATACGG175700.014.75194232
AACGACG38050.014.66414416
GACGAGG104050.014.509069
Produced by FastQC (version 0.11.5)