FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54267796
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG127250.011.33246738
GTTGTCG27550.011.10605926
GGCATAG129850.010.79235435
AGCTTAG139300.010.19227345
AACTCTA137900.010.05145
GGGAGGT224300.09.752613
GTCACTC210600.09.4817488
GGTCACT208850.09.3001167
CATAGGG152750.09.1020337
TGGCATA157650.08.97485734
GGAGGTC238150.08.9109774
TAATCGG45050.08.8917944
GCATAGG158550.08.76845636
ACTCTCC230600.08.69947411
CCATATA89300.08.6690444
TATATAC89700.08.546167
GAGCTTA167200.08.46460744
TTCCATA109850.08.3794582
GTAACGA43400.08.35045818
ACACTCG106050.08.2759554
Produced by FastQC (version 0.11.5)