FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8051973
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG23300.037.954023
ATACGGC24300.037.5689855
TACGGCG24750.037.0636756
GATACGG25650.035.934724
GCGACCA30250.030.97931510
ACGGCGA30800.030.4975367
ACCACCG32500.028.6992213
GATCTAC31100.028.5060921
GGCGACC33850.027.7496079
ATGATAC32400.027.4298842
ACCGAGA33950.027.40868616
CGACCAC34650.027.23589711
CCACCGA34550.027.12371414
CGGCGAC35400.026.534588
AGATCTA33950.026.50187320
TACACGC15400.025.28445625
CGAGATC37450.024.43609618
CACCGAG38850.024.23485215
TCTACAC37000.024.07958423
ACGACGC11800.023.30595241
Produced by FastQC (version 0.11.5)