FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6313808
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG11650.035.12186
ATACGGC11800.034.8617635
TGATACG11650.033.988843
GATACGG14150.029.2274674
GCGACCA15650.026.14498110
ACGGCGA17300.024.2871787
GATCTAC16550.024.19171121
ATGATAC17050.023.6111132
CGACCAC17650.023.30701411
CGGCGAC19350.022.282558
GGCGACC19200.021.654619
ACCGAGA18400.021.63966416
AGATCTA18850.021.47334520
CCACCGA19100.021.30728514
ACGACGC6850.020.87631441
TCTACAC20000.020.7885923
CGACGCT7050.020.59451142
ACACGAC7950.020.47838239
TACACGC8100.020.09724225
ACCACCG20950.019.95075813
Produced by FastQC (version 0.11.5)