FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6335864
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC13750.035.8362775
TGATACG13300.035.7256133
TACGGCG13900.035.29136
GATACGG15750.031.5649764
ACGACGC8450.030.46390541
ACGGCGA18000.027.9859837
GCGACCA18650.027.718310
ATGATAC18600.025.9005362
GATCTAC19100.025.79877521
CACGACG10750.024.56004540
CCACCGA20100.024.51486814
ACCGAGA19950.024.47865916
GGCGACC20300.024.3817029
CGACCAC20900.023.89225811
CGGCGAC21600.023.6271768
TACACGA11100.023.58778838
TCGTATG5350.023.4368243
ACACGAC10800.023.22439439
CGACGCT10450.022.9457142
ACCACCG21500.022.91854913
Produced by FastQC (version 0.11.5)