FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6320456
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC12400.034.5935825
TACGGCG12700.033.9496236
TGATACG12550.033.30373
GATACGG14700.029.0313364
GCGACCA16900.025.90293510
ACGGCGA17200.025.5790377
CGGCGAC18500.024.3761278
GATCTAC17900.024.3344921
ACGACGC7550.024.18645341
ACCGAGA19000.023.9662116
TCGTATG5550.023.38523143
CCACCGA18650.023.35441614
GGCGACC18500.023.3059569
CGACCAC18850.023.22332411
ATGATAC18550.022.768792
ACACGAC8300.022.53160739
CACGACG8150.021.32639340
CGTATGC6150.021.10424644
CGACGCT8550.020.8410142
TACACGA9450.020.72094338
Produced by FastQC (version 0.11.5)