FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6669246
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC86130.12914503378642803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC40450.040.6785355
TACGGCG40950.040.1818476
TGATACG39950.040.1414383
GATACGG42050.039.0784034
ACGGCGA44100.037.411487
GCGACCA46050.035.8272810
GATCTAC45800.035.44807421
ACCGAGA46650.034.896516
GGCGACC48100.034.437549
ATGATAC47000.034.307442
ACCACCG47650.034.1626113
AGATCTA47600.034.06138220
CCACCGA48650.033.64126214
CGGCGAC49600.033.6178368
CGACCAC50200.032.6901811
TACACGC19100.031.7878525
CGACGCT17100.031.51800242
TCTACAC51650.031.43172823
ACGACGC17150.031.4289441
TACACGA18650.031.26159338
Produced by FastQC (version 0.11.5)