FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6683348
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC90430.13530643623525215No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACACATCTCGTATGC68580.10261324114799947TruSeq Adapter, Index 5 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC47750.041.3241465
TGATACG47250.041.1561973
TACGGCG49000.040.314856
GATACGG49450.039.8145264
GCGACCA51950.038.27962510
ACGGCGA52600.038.0156947
GATCTAC52900.036.92849321
CGACCAC55200.036.02584511
ACCACCG55800.035.87500813
ATGATAC55250.035.316382
CCACCGA56850.035.21240614
GGCGACC57400.034.836689
ACCGAGA56950.034.80319616
AGATCTA56350.034.70634520
CGGCGAC58000.034.5900848
TCGTATG9750.033.6175843
TCTACAC59000.033.11046223
CGAGATC59900.033.05245618
ACGACGC20250.032.5941441
CCGAGAT61050.032.50191517
Produced by FastQC (version 0.11.5)