FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6676923
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC87880.13161751303706812No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACACATCTCGTATGC69120.10352073851982418TruSeq Adapter, Index 5 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG46050.041.271973
ATACGGC47500.040.9382935
TACGGCG47950.040.508226
GATACGG48750.039.8434754
ACGGCGA53500.036.88167
GATCTAC52750.036.78459521
GCGACCA53650.036.6964810
CCACCGA56050.035.04668414
ATGATAC54500.035.034372
CGACCAC56900.034.6391211
ACCACCG57250.034.50419613
CGGCGAC57350.034.444038
AGATCTA56550.034.3514220
TACACGC22050.034.32177425
ACCGAGA57400.034.2990616
GGCGACC57950.034.087419
TACACGA22000.032.80298238
TCTACAC59150.032.6929423
CGAGATC59950.032.6207118
ACGACGC20750.032.33786841
Produced by FastQC (version 0.11.5)