FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7309563
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG14450.035.6230243
ATACGGC15250.034.619775
TACGGCG15350.034.2509236
GATACGG16800.031.4256844
ACGATAC10850.031.2284932
CACGATA10900.031.08503231
CGATACA11300.029.79017333
TCGTATG11600.029.58348743
CGTATGC11700.029.33063944
CTCGTAT12000.028.964442
GATCGGA12900.027.1565111
GTCACGA12750.026.40046529
GCGACCA20550.026.33334710
ACGGCGA20800.026.12267
TCTCGTA14100.024.80860941
GATCTAC20650.024.71595821
ATCGGAA14350.024.3740652
AGTCACG13800.024.23148228
ACCGAGA21900.024.00840416
CGACCAC22950.023.77123511
Produced by FastQC (version 0.11.5)