FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7345796
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG16950.035.5591053
TACGGCG17150.035.144426
ATACGGC17400.034.8923155
TCGTATG9250.031.6285943
GATACGG19300.031.4573174
CTCGTAT10050.029.11228242
CGTATGC10350.028.26805944
ACGATAC10850.027.78261432
CACGATA10850.027.57982331
GATCTAC21550.027.35686321
GCGACCA22550.027.02110710
ACGGCGA23750.025.7484537
CGATACA11500.025.63830233
ACCGAGA24050.025.42743716
GGCGACC24250.025.1268429
GATCGGA12300.025.0761851
ATGATAC24450.024.7413732
GTCACGA12250.024.4275129
ACCACCG25650.024.18419613
CCACCGA25450.023.85564814
Produced by FastQC (version 0.11.5)