FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7313149
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC15400.037.8537945
TGATACG15800.036.0601043
TACGGCG16650.035.011926
GATACGG18700.031.0560784
CACGATA10150.029.91216531
CGATACA10500.029.33454933
ACGATAC10750.028.65215732
TCGTATG10750.028.6486343
GATCGGA11750.028.123581
CTCGTAT11000.027.99867242
CGTATGC10950.027.92504344
ACGGCGA21450.027.689867
GATCTAC21500.027.2176921
GCGACCA21900.027.12088810
GTCACGA12150.025.35032529
GGCGACC23650.025.0210429
CGACCAC24400.024.52242311
ACCACCG24350.024.39209413
CCACCGA25000.023.93388414
ATCGGAA14350.022.9944822
Produced by FastQC (version 0.11.5)