FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3846980
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3800.024.89256143
CTCGTAT4050.022.26994742
CGTATGC4350.021.74522644
GATCGGA4400.021.0312391
TCTCGTA5000.018.48006641
ACACGTC4850.018.14274213
ATCTCGT5100.018.1177140
ATCGGAA5100.018.1160622
AGTCACG5150.017.94134528
ACGGTGT5200.016.92445832
CGTCTGA5800.015.171679516
ACGTCTG6250.015.13467715
TGATACG2051.8171704E-915.0230763
CACGTCT6450.014.66538414
TACGGCG2850.014.6653836
TGCGTAC900.00436384114.6653835
ATACGGC3151.8189894E-1213.2686815
CGGTGTG7100.013.01514633
TCACGGT7100.012.70476630
GTCACGG7300.012.05530729
Produced by FastQC (version 0.11.5)