FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2945956
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34290.11639685046212501No Hit
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT31530.10702807509684463Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG15650.040.482393
ATACGGC15800.040.3765225
TACGGCG16150.039.63776
GATACGG16550.038.5467724
ACGGCGA17600.036.8720557
GCGACCA18100.035.6104210
TACACGC7200.034.52560825
GATCTAC18450.034.33930221
CCACCGA18850.033.96015514
ATGATAC18750.033.5546532
ACCGAGA19150.033.54358316
CGACGCT8100.033.4064442
ACGACGC8300.033.3977641
ACCACCG19050.033.3726613
AGATCTA19450.032.91309720
TCGTATG4800.032.54017343
CGACCAC19750.032.52398311
CGGCGAC20200.031.9083428
CACCGAG20100.031.84820615
CACGACG8650.031.79206840
Produced by FastQC (version 0.11.5)