FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2956930
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT44550.15066301873903Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34780.11762199308066137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG18350.040.519196
ATACGGC18550.039.9637375
TGATACG18250.039.897463
GATACGG19100.038.812954
TCGTATG4650.036.90023443
ACGGCGA20500.036.484237
GCGACCA21150.035.46697210
ATGATAC20900.035.3649562
CGTATGC4850.034.92677344
GATCTAC20800.034.90278621
CTCGTAT5100.034.07797242
CGGCGAC21950.034.074118
TACACGA10250.033.91403638
ACGACGC10100.033.76420641
ACCGAGA21800.033.7031216
ACCACCG22400.033.684213
GGCGACC22300.033.637969
CGACGCT9700.033.56655542
CCACCGA22550.033.2650314
ACACGAC10550.032.94965439
Produced by FastQC (version 0.11.5)