FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2949406
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT37880.12843264033503696Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37210.12616099648539403No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCGTCATCTCGTATGC31120.10551277104610217TruSeq Adapter, Index 11 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC19800.040.5500075
TGATACG19500.040.4970253
TACGGCG20250.039.7575236
GATACGG20800.038.706244
ACGGCGA22000.036.994947
GATCTAC22300.036.30304321
GCGACCA22500.036.07506610
GGCGACC22850.035.4262289
ATGATAC22550.035.2146952
CGGCGAC23200.035.081418
CGACCAC23400.035.0635811
ACCGAGA23300.035.02525316
ACCACCG23500.035.00797713
CCACCGA23650.034.8789514
ACACGAC9750.034.07579839
TACACGA9750.034.07579838
TCGTATG4600.033.47368243
TACACGC9350.033.41172825
AGATCTA24250.033.38269820
CGACGCT9650.033.2832442
Produced by FastQC (version 0.11.5)