FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2948321
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT37800.1282085634501806Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34680.11762626932413398No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCGTCATCTCGTATGC31350.10633170540114187TruSeq Adapter, Index 11 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG18500.041.141146
TGATACG18300.040.629133
ATACGGC18850.040.3772475
GATACGG20050.038.0703664
ACGGCGA20950.037.0648847
ACGACGC8750.036.7086441
GCGACCA22050.035.51512510
GATCTAC21700.035.38195421
ACCGAGA22150.035.15616616
GGCGACC22700.034.3043639
ATGATAC21750.034.0833742
ACCACCG22950.034.02652713
CGACGCT8600.034.0198642
CCACCGA23050.033.87890614
CGGCGAC23100.033.8055768
CGACCAC23100.033.7103511
TACACGA9950.033.61152638
ACACGAC9700.033.34366239
AGATCTA23050.033.30912820
TCTACAC22950.032.8796823
Produced by FastQC (version 0.11.5)