FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6208691
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85770.13814506149524916No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCTCGTATGC67730.10908901731459981TruSeq Adapter, Index 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC10100.034.413444
TGATACG7800.034.125783
TCGTATG10400.033.420743
GATCGGA11100.032.948451
ATACGGC8350.032.668345
TACGGCG8700.031.1012386
CTCGTAT11900.029.5779742
AATCTCG12650.028.17350239
GATACGG9800.028.0592334
ACACGTC13100.027.87588313
ATCGGAA13100.027.7079542
TCTCGTA13250.026.39961241
ACGTCTG14750.025.20498815
CGTCTGA16200.022.94972616
CACACGT16100.022.68161812
ATCTCGT16400.021.73138840
CACGTCT16950.021.54419114
ACGGCGA12700.021.478797
GCGACCA12850.021.05687110
TCGGAAG18000.020.5318763
Produced by FastQC (version 0.11.5)