FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6201544
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90930.1466247760235193No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCTCGTATGC72400.11674512024747385TruSeq Adapter, Index 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG8650.034.33139843
TGATACG7700.030.5677413
CGTATGC9750.030.45811344
TACGGCG8250.030.12976
ATACGGC8750.028.1566055
GATCGGA11150.028.0607131
CTCGTAT10500.027.86487842
ACGTCTG11950.026.50726315
ACACGTC11950.026.32318513
AATCTCG11350.026.16994339
ATCGGAA12050.025.9221862
TCTCGTA11750.025.27721641
GATACGG9750.025.2687474
ACGGCGA11000.022.7972497
CACGTCT14450.021.92123214
GCGACCA12000.021.63072410
GATCTAC11750.021.34434321
CACACGT14900.021.1115512
CGTCTGA15700.020.31602116
ATCTCGT16450.018.45667840
Produced by FastQC (version 0.11.5)