FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8812333
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC118900.13492454268353227TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGA16650.035.149734
CTAATCG17050.034.06699433
CGTATGC17700.032.93538744
TCGTATG18100.032.4506143
ATCGATC18050.032.4234136
AATCGAT18200.031.9144135
GATCGGA18650.031.5410481
CTCGTAT19150.030.4419342
ATCTCGT20250.029.11618440
TCTCGTA20500.028.7611141
ACACGTC21050.028.00724813
ATCGGAA21200.027.7053172
ACGTCTG22750.026.59126715
TGATACG8050.025.4141033
ATACGGC8300.025.1786965
CGTCTGA23600.025.07467816
CACGTCT24500.024.51231414
GTCACTA24150.024.23268329
ACTAATC24850.024.08223532
TACGGCG8700.024.0210576
Produced by FastQC (version 0.11.5)