FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8804683
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC125460.14249235321703235TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG16800.034.0442843
CTAATCG16800.033.13391533
CGTATGC17250.033.02920544
TAATCGA16800.033.0029534
GATCGGA18300.032.262321
CTCGTAT18550.030.95202342
ATCGATC18350.030.81526636
AATCGAT18650.030.08362635
TCTCGTA19750.028.96461341
ACACGTC20550.028.68818313
ATCGGAA21000.028.178192
ATCTCGT20550.027.7299740
ACGTCTG23000.025.82355315
CGTCTGA23500.025.36772316
TGATACG8300.024.913223
ATACGGC9150.023.8009455
CACGTCT24900.023.76473414
TACGGCG8950.023.595456
ACTAATC24000.023.5601732
GTCACTA24650.022.7593629
Produced by FastQC (version 0.11.5)