FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7569415
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGC295420.39028115118539547TruSeq Adapter, Index 2 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA125020.16516467917269698No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC122880.1623375122119741No Hit
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT77770.10274241800720399Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG57300.041.3856663
TACGGCG58350.041.357246
ATACGGC58900.041.00845
GATACGG60000.040.2199134
TCGTATG36300.039.93652743
CGTATGC36250.039.74887544
ACGGCGA63250.038.4662977
GATCGGA39100.037.756861
CTCGTAT38550.037.43490242
GCGACCA65850.037.0143210
GATCTAC64250.036.9791121
ACCACCG66300.036.59719513
CCACCGA66900.036.43337614
TCTCGTA39900.036.39124741
CGGCGAC67050.036.1878288
ACACGTC40350.036.09110613
ACCGAGA67450.035.8441916
ATGATAC66450.035.753172
GGCGACC68350.035.6604659
ATCGGAA41700.035.6084862
Produced by FastQC (version 0.11.5)