FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7599799
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGC302110.3975236713497291TruSeq Adapter, Index 2 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA127040.1671623157401926No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC123000.16184638567414744No Hit
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT122250.16085951746881727Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG66450.041.5791633
TACGGCG67800.041.3677146
ATACGGC68250.041.223895
CGTATGC38600.040.52049644
TCGTATG38850.040.20206543
GATACGG69950.040.1276744
ACGGCGA71800.039.583957
CTCGTAT40000.039.15727642
GCGACCA74250.038.24818410
GATCGGA42950.037.7060931
GATCTAC74200.037.65236721
TCTCGTA42100.037.41841
CCACCGA76600.037.0460614
CGGCGAC76900.036.901548
CGACCAC76900.036.84432611
GGCGACC77250.036.7343449
ATCGGAA44000.036.6464842
ACCACCG77500.036.5306913
ACGACGC30850.036.5144241
ACCGAGA77600.036.51196316
Produced by FastQC (version 0.11.5)