FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7584691
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGC313060.4127524773257078TruSeq Adapter, Index 2 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA129290.17046178941238346No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC128530.16945977100451423No Hit
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT96640.12741455123221238Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG69250.041.740723
ATACGGC70100.041.736695
TACGGCG69950.041.7003946
GATACGG71500.040.8271644
GCGACCA75650.039.08181410
CGTATGC36100.038.8170344
TCGTATG36200.038.7092943
ACGGCGA76700.038.5754747
GATCTAC76850.037.44205521
GATCGGA38900.037.4300731
ACCACCG79700.037.23385613
CTCGTAT37650.037.21972342
CGACCAC79650.037.1743711
CGGCGAC79700.037.151058
ACCGAGA79600.037.1424516
GGCGACC79900.037.0029959
CCACCGA80000.036.9567414
ATGATAC80000.036.2418022
ATCGGAA40600.035.9229352
TCTCGTA39250.035.87488641
Produced by FastQC (version 0.11.5)