FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7576558
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGC308830.40761253329018265TruSeq Adapter, Index 2 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA126530.16700195524141703No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC125020.1650089658127081No Hit
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT96960.12797367881299135Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG65950.041.8607983
ATACGGC68000.041.439915
TACGGCG68450.041.1674776
GATACGG69600.040.4872674
CGTATGC36200.040.16798844
TCGTATG36600.039.96914743
ACGGCGA72100.039.632597
CTCGTAT37200.039.08923342
GCGACCA75100.038.1079810
GATCGGA39550.037.7662851
TCTCGTA38600.037.73097241
GATCTAC74900.037.41972421
CCACCGA76850.037.2115814
ACCACCG77250.036.99041713
CGACCAC77500.036.92786411
CGGCGAC77700.036.8044978
ACCGAGA77700.036.74787516
ACACGTC41400.036.29113
GGCGACC79100.036.0696649
ATCGGAA41500.036.044532
Produced by FastQC (version 0.11.5)