FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4496519
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG6300.033.17271443
CGTATGC6800.030.73354544
ATACGGC3750.028.7450315
TATCTCG7150.028.6147139
CTCGTAT7350.028.43375842
GATCGGA7950.026.0522371
TACGGCG4350.025.7916376
TCTCGTA8650.025.17859841
TGATACG4250.024.3280283
ACACGTC8900.024.22334113
ATCGGAA9000.022.976472
ATCTCGT9350.021.8818440
ACGTCTG10450.020.41989315
CGTCTGA11000.020.1988516
CACGTCT10750.019.85003514
GTCACTA10550.019.60187129
GATACGG5750.018.746764
CACACGT11600.018.2058612
TCGGAAG11550.017.9037443
TAATATC12100.017.27328136
Produced by FastQC (version 0.11.5)