FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4508557
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46680.10353645301589844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT7250.031.5562942
TCGTATG7050.031.51435743
GATCGGA7500.030.261781
CGTATGC7600.029.23468244
TATCTCG8200.027.09916739
TCTCGTA8550.026.7618241
ACACGTC8700.026.04318813
ATACGGC3550.026.0253815
ATCGGAA8900.025.2107832
TACGGCG3550.024.7860776
ACGTCTG9950.022.5503515
ATCTCGT10050.021.89184440
GTCACTA11300.019.66531829
CACACGT11650.019.6373812
CACGTCT11650.019.25973914
CGTCTGA11800.019.01491216
TCGGAAG12100.018.9070643
TGATACG4600.018.6501753
CACTATA12250.018.32006331
CGGAAGA13100.017.2958584
Produced by FastQC (version 0.11.5)