FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4493778
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48030.10688111428735465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG6200.031.57762743
CGTATGC6650.029.77192144
GATCGGA6950.028.5274311
ATCGGAA7550.026.8053842
CTCGTAT7450.026.5755342
ACACGTC8400.024.09293613
TACGGCG4400.023.9977056
TGATACG4050.023.8989493
TATCTCG8150.023.48607639
ATACGGC4650.023.180585
ATCTCGT8550.022.12949440
TCTCGTA9100.021.75906641
ACGTCTG9100.021.51442515
CGTCTGA10250.020.38829416
CACGTCT10400.018.82512514
TAATATC10500.018.4394136
GTCACTA11500.017.9830929
CACACGT11050.017.91683812
GATACGG5750.017.5983184
TCGGAAG11950.017.3037853
Produced by FastQC (version 0.11.5)