FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4734023
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG4150.027.03404643
CGTATGC4550.025.14090744
CTCGTAT5600.020.03437242
GATCGGA6050.019.3044681
ACACGTC6600.016.99850313
TCTCGTA6350.016.97613741
ATCTCGT7100.015.49273840
TACGGCG2900.015.1710776
ATCGGAA7800.014.9474012
ACGTCTG8050.014.20992815
CGTCTGA8600.013.30115316
ATACGGC3203.6379788E-1213.0613495
CACGTCT9750.012.18354214
CATCTCG9150.011.78125539
CACACGT9750.011.732312
GCACACG11300.011.48571311
GATACGG3857.2759576E-1211.4275644
TGATACG3603.3105607E-1010.9990313
TTAGCGA1853.7268118E-410.70198519
ACGACGC3105.0336894E-810.6450141
Produced by FastQC (version 0.11.5)