FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4731411
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG4300.025.57899943
CGTATGC4950.021.77596944
CTCGTAT5350.020.97110742
GATCGGA5650.020.664281
TCTCGTA5850.019.1799241
ACACGTC6300.018.1570313
ATCTCGT6650.017.20339840
ATCGGAA7550.015.7336242
CACGTCT8200.014.21818114
ACGTCTG8150.014.03549615
CGTCTGA8900.013.09989616
CGCGATT1200.001943826412.8501921
ATACGGC2603.7380232E-912.6911195
TGATACG2502.9454895E-812.3188463
TCGGAAG9950.012.1596653
CACACGT10350.011.902266512
GCACACG10900.011.70532611
TAGGTCG2101.0290331E-410.4752099
CGGAAGA11850.010.395654
CATCTCG11950.010.31007439
Produced by FastQC (version 0.11.5)