FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5455088
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAGCATCTCGTATGC61080.1119688628304438TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG8550.036.53563743
CTCGTAT9400.033.23188442
CGTATGC9700.032.4311844
GATCGGA11200.030.4911251
TCTCGTA11150.028.41201241
ATACGGC6700.027.9083795
TGATACG6500.027.7517873
ATCGGAA12250.027.6550942
TACGGCG7000.027.3408286
ACACGTC13000.026.05960513
ATCTCGT12750.025.01912940
ACGTCTG14200.023.70246715
CACGTCT15000.023.0249614
CACACGT14700.022.74661312
CGTCTGA14900.022.73719416
GTCACTA14100.022.62450829
TCGGAAG15750.021.788863
GCACACG16400.021.32768611
TCACTAC15450.020.36281830
GATACGG9050.020.1752994
Produced by FastQC (version 0.11.5)