FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5449808
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAGCATCTCGTATGC62660.11497652761344986TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG9100.033.1177943
CGTATGC9550.031.55813844
CTCGTAT9800.030.97755642
GATCGGA10600.030.1449131
ATACGGC5550.027.7451635
TCTCGTA11050.027.4768441
ATCGGAA11850.026.9173222
TGATACG5900.026.4721093
ACGTCTG12700.025.11576815
ACACGTC12800.024.57583413
TACGGCG6600.023.6644616
ATCTCGT12700.023.56075140
CACGTCT14200.022.30778114
GTCACTA14100.021.84353829
CACACGT14900.021.55503812
CGTCTGA14800.021.25477416
TCGGAAG15700.020.4566923
GATACGG7750.020.152964
GCACACG16550.019.804811
TCACTAC15700.019.75793330
Produced by FastQC (version 0.11.5)